Method for producing multipolar cell

摘要

This invention provides a multipolar cell including three or more centrosomes dispersed therein and a multipolar spindle formed so as to extend from the three or more respective centrosomes. The multipolar cell production method provided by the present invention supplies cultured eukaryotic cells with a synthetic peptide as a multipolarity-inducing peptide, with the synthetic peptide having an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 10 or an amino acid sequence formed by substituting, deleting and/or adding one, two or three amino acid residues in/from/to the selected amino acid sequence. As the eukaryotic cells, cultured tumor cells are used.

主权项

1. A method for producing from at least one species of eukaryotic cells a multipolar cell having three or more centrosomes and a multipolar spindle, the method comprising
culturing target eukaryotic cells, and supplying the eukaryotic cells being cultured with a synthetic peptide consisting of an amino acid sequence selected from the following amino acid sequences that act as FtsZ inhibitors or FtsA inhibitors:(SEQ ID NO: 1)(1) SVSVGMKPSPRP,(SEQ ID NO: 2)(2) FTTSNHTSRHGS,(SEQ ID NO: 3)(3) TPSLPPTMFRLT,(SEQ ID NO: 4)(4) GPHHYWYHLRLP,(SEQ ID NO: 5)(5) QSPVNHHYHYHI,(SEQ ID NO: 6)(6) NMTTYPMHNNTV,(SEQ ID NO: 7)(7) SLLPHSNHAKHY,(SEQ ID NO: 8)(8) EFEYFHPATFRL,(SEQ ID NO: 9)(9) GPHLGMNQRRRP,and(SEQ ID NO: 10)(10) GAVTYSRISGQY.