Compositions and methods for the detection of antibodies to native human leukocyte antigen

摘要

Provided herein are compositions comprising native and denatured human leukocyte antigens (HLA) and methods of making said compositions. Also provided herein are methods and kits for the detection of antibodies to native HLAs.

主权项

1. A method of screening for antibodies that bind native human leukocyte antigens, comprising the steps of: a. contacting a sample with a composition comprising 1) a solid substrate having linked thereto purified human leukocyte antigens (HLA) wherein at least 90% of the purified HLA linked to the solid substrate are native HLA and at most 10% of the purified HLA linked to the solid substrate are denatured HLA and 2) a neutralized enzyme selected from the group consisting of a trypsin-like clan protease, trypsin, chymotrypsin and elastase, and b. detecting binding of an antibody to the solid substrate, wherein binding of an antibody to the solid substrate is indicative of a sample comprising antibodies specific for said native human leukocyte antigens.