Streptavidin mutein exhibiting reversible binding for biotin and streptavidin binding peptide tagged proteins

摘要

The present invention relates to a new streptavidin muteins. This mutein is binds both streptavidin binding peptide tagged and biotin or biotinylated molecules, and does so in a reversible fashion. As such, it is stable enough to allow reuse, and producible with reasonable production yield via secretion in a soluble functional state without the requirement of refolding via the tedious and expensive denaturation and renaturation processes.

主权项

1. A streptavidin mutein comprising a substitution of the glycine at residue 48 corresponding to full-length wild-type streptavidin (SEQ ID NO:1), wherein said substitution comprises threonine, aspartic acid, glutamic acid, asparagine or glutamine.