| 发明名称 | Cyclic RNA and protein production method | ||
| 摘要 | The present invention provides a cyclic RNA preferable for carrying out rotary protein translation in which translation domains other than that of the target protein are sufficiently short and translation efficiency is high, and a method for producing protein that uses this cyclic RNA as template. More specifically, the present invention provides a cyclic RNA that encodes a protein, has a full-length number of bases that is equal to or greater than 102 and is a multiple of 3, has at least one start codon, does not have a stop codon in the same reading frame as the start codon, and does not contain an internal ribosome entry site (IRES). In addition, the present invention provides a method for producing protein in a eukaryotic cell expression system that consists of using the aforementioned cyclic RNA as template and expressing a protein encoded by that cyclic RNA. | ||
| 申请公布号 | US9353153(B2) | 申请公布日期 | 2016.05.31 |
| 申请号 | US201314376665 | 申请日期 | 2013.02.08 |
| 申请人 | RIKEN | 发明人 | Abe Hiroshi;Abe Naoko;Ito Yoshihiro;Nishihara Mizuki |
| 分类号 | C12P21/00;C12P21/02;C07K7/06;C12N15/10;C12N15/66;C12N15/67;C12N15/70;C12N15/79;C12N15/00 | 主分类号 | C12P21/00 |
| 代理机构 | Ostrolenk Faber LLP | 代理人 | Ostrolenk Faber LLP |
| 主权项 | 1. A method for producing protein in eukaryotic cells or in a eukaryotic cell expression system, comprising: introducing a cyclic RNA into eukaryotic cells, or adding the cyclic RNA to a eukaryotic cell-free expression system; and expressing a protein encoded by the cyclic RNA using the cyclic RNA in the eukaryotic cells or eukaryotic cell-free expression system, wherein the cyclic RNA encodes a protein, has a full-length number of bases that is equal to or greater than 102, and is a multiple of 3, has at least one start codon, does not have a stop codon in the same reading frame as the start codon, and does not contain an internal ribosome entry site (IRES). | ||
| 地址 | JP | ||