| 摘要 |
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Disclosed is a method of producing compactin, involving culturing Penicillium citrinum VKPM F-1099 strain on a culture medium. Obtained culture liquid alkalised to pH 12.0 at 30-50 °C for 2-3 hours. Mycelium is then separated. Method then includes performing sorption of compactin from native solution on a column with hydrophobic nonionic sorbent with elution thereof with aqueous solution of isopropanol. Compactin is extracted from obtained solution with butyl acetate with acidification to pH 3.0-5.0. Extract is purified with activated carbon. Compactin is concentrated and lactonised with partial evaporation of solvent at residual pressure of 10-14 kPa and heating to 75-95 °C in presence of a mineral acid. Method includes crystallising compactin-lactone from aqueous solution of isopropanol at room temperature and periodic stirring for 1-2 hours, then at a temperature of 6-10 °C for at least 4 hours. Product is then recrystallised.EFFECT: invention provides degree of lactonisation of compactin of not less than 99 % and obtaining a product with compactin content of not less than 98 %, sum of impurities of not more than 1 %, single maximum dimer impurity of not more than 5/10 % and output of product of not less than 65 %.1 cl, 3 ex |
