Cas9 A method for regulation of gene expression by expressing Cas9 protein from the two independent vector

摘要

The present invention relates to a recombinant vector expressing a first domain which includes an N-terminus of a Cas9 protein, and to a gene regulation method including a step of introducing, into each cell, a recombinant vector expressing a second domain which includes a C-terminus. The present invention further relates to a composition comprising the recombinant vector, a kit for regulating the gene expression, and a method for intracellularly producing the Cas9 protein. The present invention further relates to a transgenic cell into which a viral vector packaging the first domain and a viral vector packaging the second domain are introduced. The present invention further relates to a composition comprising the virus produced therefrom.