The present invention relates to a method for promoting directed differentiation of human pluripotent stem cells into retinal pigment epithelium (RPE) fate, the method comprising: a. culturing human pluripotent stem cells in a culture system comprising a basic medium supplemented with nicotinamide (NA) to generate differentiating cells; and b. culturing said differentiating cells in a culture system comprising a basic medium supplemented with one or more members of transforming growth factor ² (TGF ²) superfamily, whereby said human pluripotent stem cells are induced to differentiate into RPE fate.
申请公布号
HK1211619(A1)
申请公布日期
2016.05.27
申请号
HK20150112372
申请日期
2015.12.16
申请人
HADASIT MEDICAL RESEARCH SERVICES & DEVELOPMENT LI
发明人
IDELSON, MASHA;ALPER-PINUS, RUSLANA;OBOLENSKY, ALEX;BANIN, EYAL;REUBINOFF, BENJAMIN EITHAN;HEMO, YITZCHAK