METHODS AND APPARATUSES FOR GENE PURIFICATION AND IMAGING

摘要

The present disclosure is directed to systems, devices and methods for nucleic acid or protein purification and imaging. A system is provided including a cartridge comprising a sample input area configured to hold a sample, comprising a plurality of hybridized complexes comprising a plurality of target molecules each hybridized with probes and a plurality of non-hybridized probes. The cartridge may also include a first binding chamber configured with first magnetic beads to receive and bind the sample, a first elution channel configured to receive the first magnetic beads and elute the sample from the first magnetic beads, a second binding chamber configured with second magnetic beads to receive and bind the sample, a second elution channel configured to receive the second magnetic beads and elute the sample from the second magnetic beads, and a binding area configured to receive the eluted sample and hold molecules for imaging.

主权项

1. A cartridge configured for purifying a hybridized target molecule sample and imaging a hybridized target molecule, comprising:
a sample input area configured to hold a target molecule sample, the sample comprising:
a plurality of hybridized complexes comprising a plurality of target molecules each hybridized with a first probe and a second probe,a plurality of non-hybridized first probes, anda plurality of non-hybridized second probes; a first binding chamber configured to receive and/or contain a first affinity matrix and to receive the sample, wherein:
the first affinity matrix is functionalized with first molecules configured to bind with the non-hybridized first probes and hybridized complexes of the sample during a first period of time; the first binding chamber is additionally configured to receive a first buffer to remove non-hybridized second probes from the sample after the non-hybridized first probes and hybridized complexes of the sample bind with the first affinity matrix; a first elution channel configured to receive the first affinity matrix after the first period of time and configured for heating the first affinity matrix to elute a first eluted sample comprising the plurality of hybridized complexes and plurality of non-hybridized first probes; a second binding chamber configured to receive and/or contain a second affinity matrix and to receive the first eluted sample, wherein:
the second affinity matrix is functionalized with second molecules configured to bind with the hybridized complexes during a second period of time; the second binding chamber is additionally configured to receive a second buffer to remove at least non-hybridized first probes; a second elution channel configured to receive the second affinity matrix after the second period of time and configured for heating the second affinity matrix to elute a second eluted sample comprising the plurality of hybridized complexes; and a binding area having an active binding surface configured to receive the second eluted sample and bind with the hybridized complexes.