CELL CULTURE METHODS TO REDUCE ACIDIC SPECIES

摘要

The instant invention relates to the field of protein production and purification, and in particular to compositions and processes for controlling the amount of acidic species expressed by host cells, as well as to compositions and processes for controlling the amount of acidic species present in purified preparations.

主权项

1. A composition comprising adalimumab, wherein the composition comprises less than 10% total acidic species of adalimumab,
wherein the acidic species of adalimumab do not include process-related impurities selected from the group consisting of host cells and lysed host cells, and wherein the acidic species of adalimumab correspond to the peaks that elute earlier than the main peak in a WCX-10 HPLC chromatogram of adalimumab.