METHODS FOR NON-TRANSGENIC GENOME EDITING IN PLANTS

摘要

Materials and methods for creating genome-engineered plants with non-transgenic methods are provided herein.

主权项

1. A method for targeted genetic modification of a plant genome without inserting exogenous genetic material comprising:
(i) providing a plant cell that comprises an endogenous gene to be modified; (ii) providing a sequence-specific nuclease comprising a sequence recognition domain and a nuclease domain; (iii) transfecting the plant cell with said sequence-specific nuclease; and (iv) inducing one or more double stranded DNA breaks (DSB) in the genome to produce a plant cell or cells having a detectable targeted genomic modification without the presence of any exogenous genetic material in the plant genome.