| 发明名称 |
METHOD FOR DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO MULTI-COMPETENT RENAL PRECURSORS |
| 摘要 |
This application relates to a method for differentiating pluripotent stem cells (PSCs) into multi-competent renal precursor cells expressing Six2. These renal precursor cells are able to differentiate into fully functional and fully differentiated podocytes. Moreover this application relates to a method for differentiating human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) into defined renal precursor cells expressing Six2 and podocytes based on linked steps of chemically defined medium inductions. |
| 申请公布号 |
US2016145578(A1) |
申请公布日期 |
2016.05.26 |
| 申请号 |
US201615010389 |
申请日期 |
2016.01.29 |
| 申请人 |
Hoffmann-La Roche Inc. |
发明人 |
Bailly Jacques;Ciampi Osele;Graf Martin;Iacone Roberto;Patsch Christoph |
| 分类号 |
C12N5/071 |
主分类号 |
C12N5/071 |
| 代理机构 |
|
代理人 |
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| 主权项 |
1. A method for differentiating pluripotent stem cells into renal precursor cells expressing SIX2, the method comprising the steps of:
a) providing a monolayer of pluripotent stem cells in a pluripotency medium b) incubating the cells in a priming medium supplemented with a small molecule inhibitor of glycogen synthase kinase 3 (Gsk3a-b), c) inducing the differentiation by incubating the primed cells in an induction medium. |
| 地址 |
Little Falls NJ US |
