| 摘要 |
The present invention is directed towards methods for producing cell and tissue compositions suitable for therapeutic applications to a mammal in need of a therapeutic cell or tissue treatment. In particular, the invention is directed towards a method of culturing cells for use in therapy, which method comprises (i) culturing a sample of cells in a first cell culture medium comprising a first semm, serum substitute and/or serum fraction and (ii) prior to harvesting cells for use in therapy culturing the cells in a second cell culture medium comprising a second serum, serum substitute and/or serum fraction. |
