SLIDE HOLDER FOR DETECTION OF SLIDE PLACEMENT ON MICROSCOPE

摘要

The invention provides a slide holder that utilizes edge detection algorithms to determine the location of various edges of a slide. From these edges, and integrating characteristic slide dimension, both linear offset of the edge of the slide in the holder and any rotation of the slide relative to the microscope stage axis are determined. The process of using the slide holder of the invention ensures precise and accurate placement of a slide during a first round of imaging and for reload in subsequent rounds.

主权项

1. A slide holder for detecting placement of a slide on a microscope comprising:
a horizontal base portion having a length, width, and height; at least two side wall portions that extend toward an opposite horizontal base portion, and together with the horizontal base form a peripheral three dimensional frame having a top planar surface and an underside planar surface, with an open imaging region formed therein; an etched groove in the top planar surface, the etched groove forming a secondary planar surface to position the slide within the peripheral three dimensional frame; one or more placement sliders secured by retractable adjustments to the peripheral three dimensional frame and adjacent a support to position the slide, wherein the retractable adjustments and the support secure the slide within bounds of the secondary planar surface of the peripheral three dimensional frame; and at least two notches configured with the secondary planar surface, a first notch to provide imaging visibility of a first location of a side edge of the slide and a second notch to provide imaging visibility of a second location at a corner of the slide when the slide holder is positioned upon an imaging microscope stage.