COMPOSITIONS AND METHODS FOR THE IMPROVED PRODUCTION AND DELIVERY OF RNA BY EFFICIENT TRANSCRIPTION TERMINATION

摘要

Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

主权项

1. An engineered expression construct comprising:
a. a promoter; b. a first nucleic acid sequence positioned transcriptionally downstream of the promoter, wherein the first nucleic acid sequence encodes a dsRNA or a protein; and c. a second nucleic acid sequence, positioned 3′ to the first nucleic acid sequence, wherein the second nucleic acid sequence forms a secondary structure comprising two or more hairpins; wherein the first nucleic acid sequence and second nucleic acid sequence are operably linked to the promoter.