Nuclease-Resistant DNA Analogues

摘要

The present invention provides stable, nuclease-resistant TNA and TNA-DNA oligonucleotides, wherein the oligonucleotides are completely resistant to enzymatic degradation for at least 24-72 hours. Methods of synthesis and use in diagnostic and therapeutic applications are also provided. Specifically, in one embodiment, we describe the chemical and biological stability of TNA and mixed-backbone (mosaic) TNA-DNA oligonucleotides under a variety of conditions and sequence contexts.

主权项

1. A stable, nuclease-resistant TNA oligonucleotide, wherein the TNA oligonucleotide comprises an effective amount of TNA and is resistant to enzymatic degradation for at least 72 hours.