| 发明名称 |
STEPWISE DIFFERENTIATION OF STEM CELLS FOR THE PRODUCTION OF EUKARYOTIC MEMBRANE PROTEINS |
| 摘要 |
A method useful for making a eukaryotic membrane protein in vitro may be carried out by (a) propagating in vitro vertebrate stem cells; (b) transforming the vertebrate stem cells in vitro with a heterologous expression vector containing a nucleic acid encoding the eukaryotic membrane protein; and then (c) differentiating the stem cells in vitro into differentiated cells (or photoreceptor-like cells) that express the membrane protein. |
| 申请公布号 |
US2016145641(A1) |
申请公布日期 |
2016.05.26 |
| 申请号 |
US201414779238 |
申请日期 |
2014.03.26 |
| 申请人 |
UAB RESEARCH FOUNDATION |
发明人 |
DELUCAS Lawrence J.;KAPPES John C. |
| 分类号 |
C12N15/85;C07K14/705;C12N5/071 |
主分类号 |
C12N15/85 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method useful for making a eukaryotic membrane protein in vitro, comprising:
(a) propagating in vitro vertebrate stem cells; (b) transforming said vertebrate stem cells in vitro with a heterologous expression vector containing a nucleic acid encoding said eukaryotic membrane protein so that said membrane protein is operatively associated in said vertebrate stem cells with a promoter; and then (c) differentiating said stem cells in vitro into differentiated cells or photoreceptor-like cells that express said membrane protein. |
| 地址 |
Birmingham AL US |
