Broad-Spectrum Monoclonal Antibody Recognizing HA1 Domain of Hemagglutinin of Influenza Virus

摘要

The invention relates to antibodies recognizing epitopes on HA1 domain of hemagglutinin (HA) protein of influenza virus, cell lines for producing the antibodies, and uses thereof. The antibodies according to the invention can specifically bind to HA1 domain of different HA subtypes and specifically bind to HA1 domain of hemagglutinin (HA) protein of H1 subtype (including seasonal H1N1 and 2009 pandemic H1N1) and H5 subtype of influenza viruses. Therefore, the invention also relates to vaccines or pharmaceutical compositions for preventing and/or treating infection of H1 subtype and H5 subtype influenza virus and/or a disease caused by the infection (e.g. influenza), comprising the antibodies according to the invention.

主权项

1. A monoclonal antibody and an antigen binding fragment thereof,
which can specifically bind to HA1 domain of different HA subtypes of influenza virus and specifically bind to HA1 domain of HA protein of H1 subtype (including seasonal H1N1 and 2009 pandemic H1N1) and H5 subtype of influenza viruses, wherein the monoclonal antibody has the heavy chain and light chain CDRs selected from the group consisting of: 1) the heavy chain CDR1-3 set forth in SEQ ID NOs: 1-3, and the light chain CDR1-3 set forth in SEQ ID NOs: 4-6; 2) the heavy chain CDR1-3 set forth in SEQ ID NOs: 7-9, and the light chain CDR1-3 set forth in SEQ ID NO: 10-12; and 3) the heavy chain CDR1-3 set forth in SEQ ID NOs: 13-15, and the light chain CDR1-3 set forth in SEQ ID NO: 16-18.