发明名称 |
MODIFIED MATRIX PROTEINS OF VESICULAR STOMATITIS VIRUS |
摘要 |
The present invention relates to vesicular stomatitis virus (VSV) matrix (M) protein mutants. One mutant M protein includes a glycine changed to a glutamic acid at position (21), a leucine changed to alanine at position (111) and a methionine changed to an arginine at position (51). Another M protein mutant includes a glycine changed to a glutamic acid at position (22) and a methionine changed to an arginine at positions (48) and (51). These new rVSVs having the mutant M are significantly attenuated and lost virulence, including neurovirulence, and are capable of inducing an immune responses against an antigen of interest. In addition, a rVSV serotype Indiana having the first described M mutant is capable of efficient replication at 31° C., and of poor replication or incapable of replication at about 37° C. or higher. |
申请公布号 |
US2016144022(A1) |
申请公布日期 |
2016.05.26 |
申请号 |
US201414901339 |
申请日期 |
2014.06.26 |
申请人 |
THE UNIVERSITY OF WESTERN ONTARIO |
发明人 |
KANG Chil-Yong;KIM Gyoung Nyoun |
分类号 |
A61K39/39;C12N7/00;C07K14/005 |
主分类号 |
A61K39/39 |
代理机构 |
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代理人 |
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主权项 |
1. A modified matrix (M) protein of a vesicular stomatitis virus (VSV), wherein the modified M protein comprises an amino acid sequence selected from: (i) SEQ ID NO: 3 including at least the following substitutions: G21E/L111A/M51R; and (ii) SEQ ID NO: 8 including at least the following substitutions: G22E/L110A/M48R/M51R. |
地址 |
London CA |