METHOD OF AMPLIFYING CIRCULAR DNA

摘要

Provided is a method capable of simply and exponentially amplifying a circular DNA, especially a long-chain circular DNA in a cell-free system. Specifically, provided is a method of amplifying a circular DNA, the method including isothermally reacting a reaction mixture which is produced by mixing a circular DNA having a replication initiation sequence (origin of chromosome (oriC)) with a reaction solution including the following enzyme groups: (1) a first enzyme group catalyzing the replication of the circular DNA; (2) a second enzyme group catalyzing a linking reaction of Okazaki fragments and synthesizing two sister circular DNAs which form catenane; and (3) a third enzyme group catalyzing a separation reaction of two sister circular DNAs.