ASSAYS FOR ANTIMICROBIAL ACTIVITY AND APPLICATIONS THEREOF

摘要

The disclosure provides methods, compositions, and kits for enhanced detection of microbes in samples and monitoring of antimicrobial activity in a subject.

主权项

1. A method of determining efficacy of an antimicrobial treatment regimen in a subject, the method comprising:
(i) assaying at least one biological sample with a pattern recognition receptor (PRR)-based assay for the presence of microbe associated molecular patterns (MAMPs), wherein the biological sample is collected from the subject who has been administered the antimicrobial treatment for no longer than 24 hours; (ii) comparing the detectable signal level of MAMPs obtained from (i) to a baseline level; and (iii) identifying the antimicrobial treatment to be effective if a treatment related change in the detectable signal level relative to the baseline level is present; or identifying the antimicrobial treatment to be ineffective if the treatment related change in the detectable signal level relative to the baseline level is absent.