| 摘要 |
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date W O 2013/096992 A1 4 July 2013 (04.07.2013) W I PO I P CT (51) International Patent Classification: (74) Agent: FB RICE; Level 23, 200 Queen Street, Melbourne, C12N 15/113 (2010.01) C12N 15/81 (2006.01) Victoria 3000 (AU). C12N 15/40 (2006.01) C12N 15/82 (2006.01) (81) Designated States (unless otherwise indicated, for every C12N 15/64 (2006.01) C12N 15/85 (2006.01) kind of national protection available): AE, AG, AL, AM, C12N15/87 (2006.01) AAOH5100 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, C12N 15180 (2006.01) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, (21) International Application Number: DO, DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, PCT/AU2012/001594 HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, (22) International Filing Date: ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, 21 December 2012 (21.12.2012) NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, (25) Filing Language: English RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, (26) Publication Language: English ZM, Zw. (30) Priority Data: (84) Designated States (unless otherwise indicated, for every 61/580,574 27 December 2011 (27.12.2011) US kind of regional protection available): ARIPO (BW, GH, (71) Applicants: COMMONWEALTH SCIENTIFIC AND GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, INDUSTRIAL RESERACH ORGANISATION UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, [AU/AU]; Limestone Avenue, Campbell, Australian Capit- TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, al Territory 2612 (AU). GRAINS RESEARCH AND DE- EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, VELOPMENT ORGANISATION [AU/AU]; Level 1, 40 MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, Blackall Street, Barton, Australian Capital Territory 2600 TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, (AU). ML, MR, NE, SN, TD, TG). (72) Inventors: WOOD, Craig Christopher; 33 Dumaresq Published: Street, Dickson, Australian Capital Territory 2602 (AU). - with international search report (Art. 21(3)) NAIM, Fatima; 46 Nungara Place, Aranda, Australian - with sequence listingpart description (Rule 5.2(a)) Capital Territory 2614 (AU). SINGH, Surinder Pal; 10 wi Lucas Place, Downer, Australian Capital Territory 2602 (AU). (54) Title: SIMULTANEOUS GENE SILENCING AND SUPRESSING GENE SILENCING IN THE SAME CELL f (57) Abstract: The present invention relates to genetically modified cells that are capable of optimal transgene expression by co-ex pressing a silencing suppressor whilst at the same time are also capable of silencing a gene, such as a naturally occurring gene of the cell. The present invention also relates to methods of producing the modified cells, as well as relates to processes for obtaining a ge netically modified cell with a desired property. |
