| 摘要 |
Reagents and methods for detection and quantitation of DNA hydroxymethylcytosine (5hmC) are provided. Specifically, genome-wide and loci analysis methods are provided that utilizes methylated primers coupled with bisulfite-sequencing for detecting and quantitating 5hmC levels in the genome. This methodology also allows quantitation of 5hmC levels at a single CpG. This method, was used to map and quantitate 5hmC sites of neurons from the human brain. This novel method can determine the exact location and abundance of 5hmC, which with bioinformatic analysis utilizing software and computers can facilitate our understanding of how modified cytosines such as hydroxymethylcytosine play a role in regulating gene expression in the brain. |
