| 摘要 |
The invention relates to methods of improving titer in transfection-based bioreactor culture production or transfection-based production systems using eukaryotic cells. A method of improving viral titer in a transfectionbased production system using a eukaryotic cell is provided. The method can include at least one of: harvesting a confluent population of eukaryotic cells that have progressed beyond log phase of cell growth for at least 24 hours prior to transfection; transfecting the cells by mixing the population with transfection reagents and plasmid DNA at the time of re-seeding the cells into a culture vessel, where the harvesting and transfecting steps, alone or in combination, results in an improved viral titer, by at least 2-fold, in a transfectionbased production using a eukaryotic cell. |
