| 摘要 |
A fluorescence observation device 1A includes a light source 11 supplying excitation light, an interlaced imaging device 12, a field memory 15 storing an image output from the imaging device 12, and a difference computing unit 20 generating a difference image. The light source 11 supplies the excitation light such that one of the first and second field image acquiring periods in the imaging device 12 is a fluorescence image acquiring period, and the other is a background image acquiring period. The difference computing unit 20 switches between a first mode of subtracting a background image of the memory 15 from a fluorescence image output from the imaging device 12, and a second mode of subtracting a background image output from the imaging device 12 from a fluorescence image of the memory 15, to apply the mode. Accordingly, in the configuration in which fluorescence observation images of an object are acquired in time series, it is possible to generate images in which fluorescence is extracted in real time. |
