| 摘要 |
Disclosed is a system and method of mass spectrometry, including: a. ionising an analyte to form a precursor ion (A) having a mass-to-charge ratio (m/z), in which m represents the mass and z the electric charge number; b. activating the precursor ion (A) by interaction with a beam of neutral species, ions, electrons or photons, having an energy chosen on the basis of the physicochemical properties of the precursor ion, the activation being suitable for producing a product ion (B, C) having the same mass m as the precursor ion (A) and an electric charge number z′ such that z′ is a non-zero integer different from z; c. separating the product ion (B, C, E, F) having a predefined mass-to-charge ratio (m/z′); d. detecting the product ion (B, C) having the predefined mass-to-charge ratio (m/z′). |
| 主权项 |
1. A method for mass spectrometry measurement of molecular species in a complex mixture, the method comprising the following steps:
a. ionising an analyte in complex mixture to form a precursor ion (A, D) of an ionised species of interest and at least one interfering ion coming from the complex mixture, the precursor ion (A, D) of said ionised species of interest and said at least one interfering ion having a same mass-to-charge ratio m/z, where m represents the mass and z the electric charge number of the precursor ion (A, D) of said ionised species of interest; b. activating the precursor ion (A, D) of said ionised species of interest and of the at least one interfering ion of same mass-to-charge ratio, by interaction with a beam of neutral species, ions, electrons or photons, having a predetermined energy as a function of the energy of ionisation of the precursor ion (A, D) of said ionised species of interest or of the energy required for the detachment of electron from the precursor ion (A, D) of said ionised species of interest, said activation being adapted to produce a product-ion (B, C, E, F), by charge transition with no fragmentation of the precursor ion (A, D) of said ionised species of interest, the product-ion (B, C, E, F) having the same mass m as the precursor ion (A, D) of said ionised species of interest and an electric charge z′ such that z′ is a non-zero integer number different from z; c. separating the product-ion (B, C, E, F) of the ionised species of interest having said predetermined mass-to-charge ratio m/z′ specific to the ionised species of interest; d. selectively detecting the product-ion (B, C, F, E) of the ionised species of interest having said predetermined mass-to-charge ratio m/z′ specific to the ionised species of interest. |
