YEAST WITH INCREASED BUTANOL TOLERANCE INVOLVING CELL WALL PROTEINS

摘要

Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. The yeast host cells provided herein comprise at least one genetic modification in a pyruvate decarboxylase gene and at least one genetic modification in an endogenous cell wall protein, which confers resistance to butanol and increased glucose utilization.

主权项

1. A yeast microorganism comprising a pyruvate utilizing biosynthetic pathway, wherein the microorganism further comprises:
a) at least one genetic modification in an endogenous cell wall protein gene; b) at least one genetic modification in an endogenous pyruvate decarboxylase gene; andwherein the microorganism has an increase in tolerance to butanol as compared to a microorganism that lacks the at least one genetic modification.