发明名称 |
METHODS AND KITS FOR POST-IVT RNA PURIFICATION |
摘要 |
The disclosure relates to methods for nucleic acid purification, comprising (a) combining a sample comprising at least one nucleic acid with a binding buffer comprising at least one magnetic particle and having a pH ranging from about 4 to about 10 to form a solution; (b) incubating the sample with the binding buffer for a time period sufficient to reversibly bind the at least one nucleic acid to the at least one magnetic particle to form at least one modified magnetic particle, (c) separating the at least one modified magnetic particle from the solution, (d) washing the at least one modified magnetic particle with at least one wash buffer; and (e) combining the at least one modified magnetic particle with an elution buffer Kits comprising these buffers and magnetic particles are also disclosed herein. |
申请公布号 |
WO2016077294(A1) |
申请公布日期 |
2016.05.19 |
申请号 |
WO2015US59867 |
申请日期 |
2015.11.10 |
申请人 |
CORNING INCORPORATED |
发明人 |
HSIEH, YI-CHENG;HSU, CHENG-I;WU, CHIA-LING;YEN, HSAN JAN |
分类号 |
C12N15/10 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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