PROCESS FOR PRODUCING HIGH PURITY FIBRINOGEN AND THROMBIN FOR FIBRIN SEALANT

摘要

The present invention relates to a fibrin sealant kit comprising purified fibrinogen and thrombin. The invention particularly relates to an improved chromatographic process for the purification of thrombin and fibrinogen components devoid of any significant plasminogen and other impurities. The absence of plasminogen facilitates the exclusion of a proteolytic inhibitor (aprotinin) from among the kit components.

主权项

1. An integrated method for the isolation and purification of plasma derived fibrinogen and thrombin, without the use of ethanol precipitation, comprising subjecting plasma to gel filtration chromatography to obtain three fractions (Fraction I, Fraction II and Fraction III) which are subjected to further all-chromatographic steps followed by viral inactivation.