PROTEIN PRODUCTION IN PLANTS

摘要

The present disclosure relates to methods and constructs for producing heterologous peptides and proteins in plants in a safe and controlled manner. One aspect of the present invention provides a method of producing heterologous protein in a transformed potato plant using an expression cassette comprising a gene coding for a protein or peptide of interest and a marker gene, a nucleotide sequence capable of suppressing patatin expression, along with a nucleotide sequence capable of suppressing CD4B expression, and/or a nucleotide sequence capable of overexpressing P19. Another aspect of the invention provides a method of producing a heterologous protein in a transformed potato plant using an expression cassette comprising a gene coding for a protein or peptide of interest, a marker gene, a transit peptide sequence, and a nucleotide sequence capable of suppressing ADP glucose pyrophosphorylase expression.

主权项

1. A method of producing a heterologous protein in a transformed potato plant comprising the steps of:
a. transforming a plant with an expression cassette, the expression cassette comprising (i) a nucleotide sequence capable of suppressing patatin expression; and (ii) a nucleotide sequence capable of suppressing CD4B expression; (iii) a marker gene and (iv) a gene coding for a protein or peptide of interest; b. cultivating the transformed plant under a defined condition; and c. extracting the protein of interest.