METHOD FOR IDENTIFYING SPECIES USING MOLECULAR WEIGHTS OF NUCLEIC ACID CLEAVAGE FRAGMENTS

摘要

A method for species identification by using molecular weights of nucleic acid cleavage fragments, comprising steps of: performing a polymerase chain reaction and a nucleic acid cleavage reaction to cleave the nucleic acid sequence of the to-be-identified species into multiple to-be-tested nucleic acid cleavage fragments having different molecular weights; measuring the molecular weights of the to-be-tested nucleic acid cleavage fragments by using a mass spectrometer; comparing the molecular weight of each of the to-be-tested nucleic acid cleavage fragments of the to-be-identified species with molecular weights of multiple known nucleic acid cleavage fragments of a known species prestored in a database; determining a number N of the identical nucleic acid cleavage fragments between the two species; and calculating a ratio N/M of the number N of the determined identical nucleic acid cleavage fragments to the total number M of the known nucleic acid cleavage fragments of the known species, wherein the ratio N/M represents similarity of the nucleic acid sequences between the to-be-identified species and the known species.