抗原負荷した樹状細胞ワクチンを前駆体から発生させる迅速ワンステップ法

摘要

The invention relates to antigen loaded antigen-presenting cells made by contacting monocytes ex vivo with soluble or particulate antigenic material, tumor necrosis factor alpha, and granulocyte-macrophage colony stimulating factor to form antigen loaded antigen-presenting cells, wherein said antigen-presenting cells comprise one or more subsets selected from the group consisting of Langerhans cells with surface markers (CD1a+ CD14- CD207+); interstitial dendritic cells with surface markers (CD1a+ CD14+ CD207-); double-negative dendritic cells (DN-DCs) with surface markers (CD1a- CD14-); and dendritic cells with surface markers (CD1a- CD14+ CD209+). Said antigen-presenting cells can be used directly as a vaccine or for producing cytotoxic cells that are used for stimulating immune response in a patient.