Method for producing a recombinant protein using a cell line adapted to a protein-free and lipid-free medium

摘要

A method for producing a recombinant protein includes steps of: (a) culturing a transformed cell in a protein-free and lipid-free medium containing no exogenous growth factors, in which the transformed cell is produced by transfecting a cell of a cell line derived from Chinese Hamster Ovary (CHO) cells, the cell line is adapted to a protein-free and lipid-free medium, and the cell is capable of proliferating in a suspended state in a protein-free and lipid-free medium containing no exogenous growth factors, with a vector containing a gene coding for the protein to be produced under the control of a promoter operable in the cell, and (b) recovering the protein produced by the transformed cell.

主权项

1. A method for producing a recombinant protein comprising:
(a) culturing a transformed cell in a protein-free and lipid-free medium comprising no exogenous growth factors,
wherein the transformed cell was produced by transfecting a cell of a cell line derived from Chinese Hamster Ovary (CHO) cells with a vector comprising a gene coding for the recombinant protein to be produced under the control of a promoter operable in the cell, andwherein the cell line is adapted to a protein-free and lipid-free medium, and is able to proliferate in a suspended state in the protein-free and lipid-free medium comprising no exogenous growth factors without altering a growth rate and cell morphologies for at least three passages, and (b) recovering the recombinant protein produced by the transformed cell.