The present invention provides a mutagenesis method wherein a nucleic acid molecule is mutagenized with at least one mutagenesis primer in a primer extension reaction and subsequently amplified by rolling circle amplification (RCA). The method involves steps leading to selective amplification of only the mutated strand by a strand-displacing DNA polymerase. Multiple copies of the mutated plasmids are generated during multiple-primed RCA and the resulting DNA is transformed for use. The method is suitable for mutating both single-stranded and double-stranded DNA. The present invention also provides a kit for use in the mutagenesis method.
申请公布号
DK2516643(T3)
申请公布日期
2016.05.17
申请号
DK20100838753T
申请日期
2010.12.21
申请人
TURUN YLIOPISTO
发明人
HUOVINEN, TUOMAS;LAMMINMÄKI, URPO;BROCKMANN, EEVA-CHRISTINE;VEHNIÄINEN, MARKUS