Antibodies to endoplasmin and their use

摘要

Isolated monoclonal antibodies are disclosed herein that specifically bind endoplasmin. In some embodiments these antibodies are fully human. Recombinant nucleic acids encoding these antibodies, expression vectors including these nucleic acids, and host cells transformed with these expression vectors are also disclosed herein. In several embodiments the disclosed antibodies are of use for detecting and/or treating tumors that express endoplasmin, such as melanoma, breast cancer, head and neck squamous cell carcinoma, renal cancer, lung cancer, glioma, bladder cancer, ovarian cancer or pancreatic cancer. In one example, the tumor is a melanoma.

主权项

1. An isolated fusion protein comprising a human monoclonal antibody or an antigen-binding fragment thereof comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region comprises amino acids 26-33of SEQ ID NO: 1 (CDR1), amino acids 51-58 of SEQ ID NO: 1 (CDR2), amino acids 97-103 of SEQ ID NO: 1 (CDR3) and wherein the light chain variable region comprises amino acids 27-32 of SEQ ID NO: 2 (CDR1), amino acids 50-52 of SEQ ID NO: 2 (CDR2), amino acids 89-97 of SEQ ID NO: 2 (CDR3), wherein the human monoclonal antibody or antigen binding fragment thereof specifically binds human endoplasmin, and wherein the human monoclonal antibody or antigen binding fragment thereof is linked to an effector molecule, wherein the effector molecule is a cytokine or a chemokine.