| 发明名称 | Recombinant yeast and substance production method using the same | ||
| 摘要 | Substance productivity is improved by introducing a metabolic pathway for synthesis of acetyl-CoA or acetic acid from glucose-6-phosphate into yeast. Acetic acid productivity, acetyl-CoA productivity, and productivity of a substance made from acetyl-CoA-derived are improved by attenuating genes involved in the glycolytic system of yeast and introducing a phosphoketolase gene into the yeast. | ||
| 申请公布号 | US9340793(B2) | 申请公布日期 | 2016.05.17 |
| 申请号 | US201113979537 | 申请日期 | 2011.01.20 |
| 申请人 | TOYOTA JIDOSHA KABUSHIKI KAISHA | 发明人 | Muramatsu Masayoshi;Ito Masakazu |
| 分类号 | C12N1/12;C12N15/00;C12N1/20;C12N9/04;C12N9/88;C12N9/90;C12P7/00;C12P7/54;C12P7/04;C07H21/04;C12N15/81;C12P7/02;C12N9/12;C12N15/52;C12P19/32;C12P7/62 | 主分类号 | C12N1/12 |
| 代理机构 | Sughrue Mion, PLLC | 代理人 | Sughrue Mion, PLLC |
| 主权项 | 1. A recombinant, non-xylose fermenting Saccharomyces cerevisiae, which comprises an attenuated phosphofructokinase gene endogenous to said Saccharomyces cerevisiae and an introduced phosphoketolase gene, wherein the expression of a Saccharomyces cerevisiae glucose-6-phosphate dehydrogenase gene and/or a Saccharomyces cerevisiae D-ribulose-5-phosphate-3-epimerase gene is enhanced, wherein acetic acid production by said recombinant Saccharomyces cerevisiae is enhanced compared with the wild-type strain, and wherein said introduced phosphoketolase gene encodes the amino acid sequence of any one of SEQ ID NOs: 1-19. | ||
| 地址 | Aichi JP | ||