ENHANCED SENSITIVITY OF DETECTION IN ELECTROSPRAY IONIZATION MASS SPECTROMETRY USING POST-COLUMN MODIFIER AND MICROFLUIDIC DEVICE

摘要

PROBLEM TO BE SOLVED: To provide a microfluidic liquid chromatography-electrospray ionization (LC-ESI) device for enhancing the sensitivity of mass spectrometric detection of an analyte in a sample.SOLUTION: The device is designed to drive effective intermixing of an analytical flow stream exiting a chromatographic stationary phase and a post-column modifier reagent. The mixed flow stream thus obtained is used for generating an electrospray containing analyte ions. Also provided are methods for enhanced sensitivity of detection of an analyte in a sample.SELECTED DRAWING: Figure 4