| 摘要 |
A nucleic acid detection board (1) and a detection system combining piezoelectric sensing and loop-mediated isothermal amplification. The nucleic acid detection board (1) comprises a piezoelectric sensor (2) and at least one pipeline (3) that passes through the surface of the piezoelectric sensor (2). Two valves (4, 5) are disposed at an interval at an upstream end of the pipeline (3) opposite to the piezoelectric sensor (2). Nucleic acid to be detected is locked in the pipeline (3) between the two valves (4, 5) for isothermal amplification before passing through the piezoelectric sensor (2). The nucleic acid detection system comprises the nucleic acid detection board (1), a thermostat (9) that can accommodate the nucleic acid detection board (1), and a signal processor (10) that can be in a data connection to the piezoelectric sensor (2). Because isothermal amplification and piezoelectric sensing are combined for joint detection, a device structure is simplified, and detection efficiency is improved. A structure that is closed all the time can avoid problems that may be involved such as aerosol contamination in nucleic acid detection, and is easy to implement batch production. The detection system is applicable to resolving existing problems of long detection time, low flux, high costs, complex steps, and the like that are caused by blood pathogens and especially caused by platelet bacterial contamination. |
