发明名称 |
Use of template switching for DNA synthesis |
摘要 |
A method of preparing a DNA copy of a target polynucleotide using template switching is described. The method includes mixing a double stranded template/primer substrate made up of a DNA primer oligonucleotide associated with a complementary oligonucleotide template strand with a target polynucleotide in a reaction medium and adding a suitable amount of a non- retroviral reverse transcriptase to the reaction medium to extend the DNA primer oligonucleotide from its 3' end to provide a DNA copy polynucleotide. The DNA copy polynucleotide includes a complementary target DNA polynucleotide that is synthesized using the target polynucleotide as a template. Methods of adding nucleotides to the double stranded template/primer substrate are also described. The method can be used to facilitate detection, PCR amplification, cloning, and determination of RNA and DNA sequences. |
申请公布号 |
AU2012220596(B2) |
申请公布日期 |
2016.05.12 |
申请号 |
AU20120220596 |
申请日期 |
2012.02.23 |
申请人 |
BOARD OF REGENTS |
发明人 |
LAMBOWITZ, ALAN M.;MOHR, SABINE;WHITE, TRAVIS B.;KUERSTEN, SCOTT |
分类号 |
C12N15/10;C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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