SCREENING OF NUCLEIC ACID AGENTS VIA PARTICLE DISPLAY

摘要

The present disclosure provides a method for identifying one or more nucleic acid agents, e.g., aptamers, having a desired property from a mixture of candidate nucleic acid agents. The method generally includes immobilizing the mixture of candidate nucleic acid agents onto particles, wherein only a subset of the candidate nucleic acid agents are immobilized on any one of the particles, and wherein the subset is present in multiple copies. The particles are exposed to a target, and particles including candidate nucleic acid agents having the desired property are isolated. In this way, one or more nucleic acid agents having the desired property may be identified. Related compositions and nucleic acid agents identified as having one or more desired properties are also provided.

主权项

1. A method for identifying one or more nucleic acid agents having one or more desired properties from a mixture of candidate nucleic acid agents, wherein the mixture of candidate nucleic acid agents comprises a plurality of single stranded nucleic acids, the method comprising:
immobilizing the mixture of candidate nucleic acid agents onto aplurality of particles, wherein only a subset of the candidate nucleic acid agents are immobilized on any one of the plurality of particles, and wherein the subset is present in multiple copies;
exposing the plurality of particles to a target, isolating a particle or particles from the plurality of particles, wherein the isolated particle or particles comprise one or more candidate nucleic acid agents having the one or more desired properties; and identifying one or more nucleic acid agents having the one or more desired properties from the isolated particles.