主权项 |
1. A method for producing a hypo-metallated redox-active metallothionein protein, the hypo-metallated redox-active metallothionein protein having 20 cysteine sulfhydryl groups and 7 binding pockets, 2 to 16 of the 20 cysteine sulfhydryl groups being free and reduced, and 1 to 6 of the 7 binding pockets being occupied by metal ions, comprising the following steps:
providing a metallothioneinprotein; de-metallating the metallothioneinprotein; chemically reducing all the 20 cysteine sulfhydryl groups of the de-metallatedmetallothionein protein; and partiallymetallating the reduced de-metallatedmetallothionein protein by providing 1, 2, 3, 4, 5, or 6 metal ions to the 7 binding pockets. |