AUTOFOCUS ALGORITHM FOR MICROSCOPY SYSTEM BASED ON CROSS-CORRELATION

摘要

Optimized automatic focusing of a microscope objective based on a cross correlation between a representative focus metric scan and a focus metric scan of a sample to be imaged.

主权项

1. A method of automatically focusing a microscope objective, the method comprising:
determining a focus position between the microscope objective and a reference microwell at a reference position on a microplate; generating an original focus metric scan based on the focus position; generating a current focus metric scan of a microwell to be imaged on the microplate using the focus position; performing cross correlation between the original focus metric scan and the current focus metric scan; and determining where along the focus axis to image a sample in the microwell to be imaged using the focus of the microscope objective based on a result of the cross correlation.