MATERIALS AND METHODS FOR RAPID AND SPECIFIC DETECTION OF COCAINE

摘要

The invention pertains to a rapid and specific aptamer-based method for one-step cocaine detection with minimal reagent requirements based on an aptamer sensor that reports the presence of cocaine via the displacement and unquenching of a bound fluorophore molecule. In certain embodiments, the invention provides novel aptamers, which have reduced background fluorescence, bind a fluorophore molecule tightly, and show an increased signal gain in the presence of cocaine.

主权项

1. A method for detecting cocaine in a biological sample wherein said method comprises contacting said sample with an aptamer to which ATMD is bound and determining whether an increase in fluorescence occurs, wherein an increase in fluorescence is indicative of the presence of cocaine in the sample.