发明名称 |
PEPTIDE-MEDIATED DELIVERY OF RNA-GUIDED ENDONUCLEASE INTO CELLS |
摘要 |
A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs. |
申请公布号 |
WO2016073433(A1) |
申请公布日期 |
2016.05.12 |
申请号 |
WO2015US58760 |
申请日期 |
2015.11.03 |
申请人 |
E. I. DU PONT DE NEMOURS AND COMPANY |
发明人 |
FRISCH, RYAN L.;FAN, XIAOCHUN;HONG, SEUNG-PYO;JACKSON, ETHEL NOLAND |
分类号 |
C12N9/22;C12N15/62 |
主分类号 |
C12N9/22 |
代理机构 |
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