| 摘要 |
A free-flow electrophoresis method for separating at least one analyte from a mixture of analytes is disclosed. The method comprises
- flowing a separation medium through a separation chamber in a flow direction;
- applying an electric field in the separation medium across an anode and a cathode, wherein the anode and the cathode are located at a distance from each other and the separation medium flows between the anode and the cathode - applying the mixture of analytes to the separation medium, whereupon the at least one analyte of interest is separated from the mixture of analytes,
- collecting fractions of the separation medium with at least one fraction comprising the analyte of interest. The separation medium comprises two or more individual separation media, which differ in their pH value and form a pH step gradient between the anode and the cathode. |
