发明名称 |
METHODS, DEVICES, AND SYSTEMS FOR BONE TISSUE ENGINEERING USING A BIOREACTOR |
摘要 |
An anatomically-shaped, human bone graft may be cultivated ex vivo using a bioreactor capable of perfusing large complex porous scaffolds. Scaffolds derived from image-based modeling of a target are seeded with human mesenchymal stem cells and cultivated. A bioreactor configured to house complex three-dimensional scaffold geometries provides controlled flow for perfusion of the cells. Dense uniform cellular growth can be attained throughout the entire scaffold as a result of the medium perfusion. In an embodiment, the bioreactor has a mold into which perfusion medium is pumped under pressure and multiple ports through which the medium exits the mold. |
申请公布号 |
EP2408401(B1) |
申请公布日期 |
2016.05.11 |
申请号 |
EP20100749299 |
申请日期 |
2010.03.03 |
申请人 |
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK |
发明人 |
VUNJAK-NOVAKOVIC, GORDANA;GRAYSON, WARREN, L.;YEAGER, KEITH |
分类号 |
A61F2/28;A61F2/30;C12M1/00;C12M1/12;C12M3/00 |
主分类号 |
A61F2/28 |
代理机构 |
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代理人 |
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地址 |
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