| 摘要 |
There is provided a method of avoiding deterioration of the accuracy in the number of detected light-emitting particles due to that two or more light-emitting particles are encompassed at a time in the light detection region in the scanning molecule counting method using an optical measurement with a confocal microscope or a multiphoton microscope. In the inventive optical analysis technique, in the detection of an individual signal indicating light of a light-emitting particle in a manner that a signal having an intensity beyond a threshold value is selectively detected as a signal indicating light of a light-emitting particle in light intensity data produced through measuring light intensity during moving the position of a light detection region in a sample solution by changing the optical path of the optical system of the confocal microscope or multiphoton microscope, the threshold value is set so that a signal indicating light from a light-emitting particle encompassed in a region narrower than the light detection region in the light detection region will be detected selectively. |
