High throughput transfection of filamentous fungi

摘要

The present invention provides a method for the transfection of filamentous fungal cells, comprising providing a multitude of containers, filling into each container an amount of polymer needed for the transfection, filling the cells to be transfected as well as an aqueous solution of transfection reagent into each of the containers, incubating the resulting mixture, removing the transfection reagent from the incubated mixture; and selecting the cells which have been transformed, characterized in that the total volume of the incubating mixture is less than 1 ml per container. Furthermore, the present invention provides the use of transformed filamentous fungal cells for the production of proteins or metabolites.

主权项

1. A high throughput method for transfection of filamentous fungal cells, comprising:
(a) providing a multitude of containers; (b) filling into each container an amount of polymer needed for the transfection; (c) filling the cells to be transfected as well as an aqueous solution of transfection reagent into each of the containers; (d) incubating the resulting mixture; (e) optionally removing the transfection reagent from the incubated mixture; and (f) selecting the cells which have been transformed,characterized in that the total volume of the incubating mixture is less than 1 ml per container, wherein said polymer of step (b) is DNA or RNA, and wherein said transfection reagent of step (c) is polyethylene glycol 4000, and wherein the aqueous solution of step (c) contains not more than 30% of polyethylene glycol.