Alpha-amylase variants

摘要

Variants of Bacillus sp. no. 707 alpha-amylase are provided that are produced more efficiently and thus more economically. Higher fermentation yields are achieved through introducing amino acid variations that promote solubility of the variant in a fermentation broth. Increased solubility allows more enzyme to remain in solution after expression in a host cell. This in turn increases the efficiency with which the expressed variant enzyme can be recovered from the fermentation broth.

主权项

1. An isolated recombinant protein comprising an amino acid sequence having at least 97% amino acid sequence identity to SEQ ID NO: 1, and comprising a substitution corresponding to A186G, using SEQ ID NO: 1 for numbering, wherein the amino acid sequence has α-amylase activity.