Podocalyxin-like-protein-1 binding antibody molecule

摘要

The disclosure relates to novel markers of pluripotent stem cells and uses thereof, and particularly, though not exclusively, to antibody molecules based on fragments of mAb84 which bind to undifferentiated pluripotent stem cells via podocalyxin-like protein-1 (PODXL).

主权项

1. An antibody molecule which binds PODXL and includes two monomers, each monomer including an Fv antibody fragment connected via a flexible linker to a dimerizing portion comprising a dimerization domain, wherein the dimerizing portions interact to form the antibody molecule, and wherein the antibody molecule is a multimer,
wherein each Fv antibody fragment has a VH region and a VL region,and comprises CDR amino acid sequences (i) to (vi):(i) VL CDR1:(SEQ ID NO: 2)SASSSVNYMY;(ii) VL CDR2:(SEQ ID NO: 3)DTSNLAS;(iii) VL CDR3:(SEQ ID NO: 4)QQWSSYPYT;(iv) VH CDR1:(SEQ ID NO: 5)NYWMN;(v) VH CDR2:(SEQ ID NO: 6)EIRLKSNNYATHYAESVKG;(vi) VH CDR3:(SEQ ID NO: 7)ERA; wherein the dimerization domain is selected from a helix-turn-helix (HTH) motif, a coiled-coil motif, or an EF hand motif; and wherein the antibody molecule is a cytotoxic antibody molecule.