| 发明名称 | CHO expression system | ||
| 摘要 | The present invention is within the field of industrial protein production. The inventors have designed and constructed a new expression system comprising an expression vector coding for a glutamine synthetase of human or dog origin, and a CHO cell line. More specifically, the invention pertains to a combination of (i) a DNA vector suitable for production of a recombinant protein, wherein said vector comprises a sequence coding for a glutamine synthetase, and (ii) a Chinese Hamster Ovary (CHO) cell line, wherein said GS comprises a sequence at least 94.5% identical to the sequence of SEQ ID NO: 1 or to the sequence of SEQ ID NO: 2. | ||
| 申请公布号 | US9334489(B2) | 申请公布日期 | 2016.05.10 |
| 申请号 | US201314407836 | 申请日期 | 2013.06.14 |
| 申请人 | SANOFI | 发明人 | Devaud Catherine;Dumas Bruno;Lounis Nabil |
| 分类号 | C12N15/85;C12P21/00;C12N9/00;C07K16/00 | 主分类号 | C12N15/85 |
| 代理机构 | McDonnell Boehnen Hulbert & Berghoff LLP | 代理人 | McDonnell Boehnen Hulbert & Berghoff LLP |
| 主权项 | 1. A Chinese Hamster Ovary (CHO) cell line comprising a deoxyribonucleic acid (DNA) expression vector, and wherein said vector comprises: a nucleotide sequence coding for a mammalian glutamine synthetase (GS) comprising a sequence at least 80% identical to the sequence of SEQ ID NO: 8 or SEQ ID NO: 9 operably linked to a heterologous promoter; and at least one expression cassette for expressing a recombinant protein, wherein said GS comprises a protein sequence at least 94.5% identical to the sequence of SEQ ID NO: 1 or to the sequence of SEQ ID NO: 2. | ||
| 地址 | Paris FR | ||